HSC transplantation, printed
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HLA typing by cellular assay

The CTLpf assay estimates the frequency of alloreactive patient-specific CTL precursors in the blood of the donor. The test is based on the recognition of these incompatibilities by the donor cytotoxic T cells mixed with the patient's cells in culture. This recognition in vitro that mimicks the recognition in vivo that causes GVHD can be used to detect class I incompatibilities that are not recognized by serology.