HLA typing by molecular techniques

Molecular polymerase chain reaction (PCR) based HLA typing techniques allow the determination of DNA sequence variations, either by hybridisation with sequence-specific oligonucleotide probes (PCR-SSO), or by a combination of sequence specific primers (PCR-SSP), or by direct sequencing (PCR-SBT) of PCR product.

Sequence-specific oligonucleotides (SSO)

Sequence-specific primers (PCR-SSP)

Sequence based typing (PCR-SBT)